Development of new RP-HPLC method for simultaneous estimation of Luliconazole and Naproxen sodium in the formulated gel

Abstract

The objective of the study was to develop a new simple, accurate, sensitive, reproducible RP-HPLC method for the simultaneous estimation ofLuliconazole and Naproxen Sodium in formulated gel.The resolution of two drugs was achieved on enable Cl8 (4.6x 150mm, 5µm) column using mobile phase phosphate buffer pH 5.8 : methanol in the ratio 60:40v/v with isocratic elution, flow rate lmL/min.Retention time ofLuliconazole and Naproxen Sodium were4.2 min and 6.8 min respectively, using analytical wavelength 220 nm. The percentage recovery for Luliconazole and Naproxen Sodium was found to be 97.8% and 99.3% respectively. The method was validated according to the ICH guidelines and it can be used for routine analysis in simultaneous estimation ofluliconazole and naproxen sodium in pharmaceutical gel.