A Simple Spectrophotometric Determination of Abacavir Sulphate in Pharmaceutical Formulations

Abstract

Two simple, sensitive and economical spectrophotometric methods have been developed for the determination of abacavir in commercial dosage forms. The method A was based on the formation of chloroform extractable complex of abacavir sulphate with wool fast blue. The absorbance of the extractable ion pair complex is measured at the wavelength of maximum absorbance 590 nm against the reagent blank. Method B was based on the charge transfer reactions of abacavir sulphate as n-electron donor with acceptor, 2,5-dichloro-3,6-dihydroxy-1,4-benzoquinone. The absorbance of the highly intensive coloured solution was measured at 450 nm against reagent blank treated similarly. Statistical analysis proves that the proposed methods are reproducible and selective for the estimation of abacavir sulphate in bulk drug and in its tablet dosage form.