Anti-microbial and Free Radical Scavenging Activity of Chamomile Flower Essential Oil

Authors

  • S.P.S. Bisht Department of Biotechnology, Roland Institute of Pharmaceutical Sciences Berhampur-760010, Orissa, India Author
  • Rojita Mishra Department of Biotechnology, Roland Institute of Pharmaceutical Sciences Berhampur-760010, Orissa, India Author
  • Kasturi Kumari Department of Biotechnology, Roland Institute of Pharmaceutical Sciences Berhampur-760010, Orissa, India Author

DOI:

https://doi.org/10.5530/b8fa8382

Keywords:

Matricaria chamomilla, Gas chromatographic technique, DPPH, á-bisabolol oxides A, Chamazulene, Matricaria chamomilla is in use for last many years as herbal remedy in various countries it is a well known herbal plant in ancient Egypt, Greece, and Rome. The chamomile drug is included in the Pharmacopoeia of 26 countries. The present study was focused on the characterization of the main constituents of Chamomile essential oil, its antioxidant property and antimicrobial activity. The essential oil and plant extracts was extracted by Clevenger apparatus and by Soxhlet apparatus respectively with various solvents. The compound analysis of Chamomile essential oil was analysed by Gas chromatographic technique. After component analysis the antioxidant activity of methanolic extract was performed using DPPH assay. The antibacterial assays were carried out by the disc-diffusion in order to determine the antibacterial activity of oil against the strains Bacillus subtilis (ATCC 10707), Escherichia coli (ATCC 0157:H7), Proteus vulgaris (MTCC 426), and Pseudomonas aeruginosa (ATCC 27853). This oil was found as a good source of á-bisabolol oxides A and chamazulene which are primarily responsible for antioxidant activity and antimicrobial activity of this essential oil.

Abstract

Matricaria chamomilla is in use for last many years as herbal remedy in various countries it is a well known herbal plant in ancient Egypt, Greece, and Rome. The chamomile drug is included in the Pharmacopoeia of 26 countries. The present study was focused on the characterization of the main constituents of Chamomile essential oil, its antioxidant property and antimicrobial activity. The essential oil and plant extracts was extracted by Clevenger apparatus and by Soxhlet apparatus respectively with various solvents. The compound analysis of Chamomile essential oil was analysed by Gas chromatographic technique. After component analysis the antioxidant activity of methanolic extract was performed using DPPH assay. The antibacterial assays were carried out by the disc-diffusion in order 
to determine the antibacterial activity of oil against the strains Bacillus subtilis (ATCC 10707), Escherichia coli (ATCC 0157:H7), Proteus vulgaris (MTCC 426), and Pseudomonas aeruginosa (ATCC 27853). This oil was found as a good source of á-bisabolol oxides A and chamazulene which are primarily responsible for antioxidant activity and antimicrobial activity of this essential oil. 

 

 

 

 

Author Biographies

  • S.P.S. Bisht, Department of Biotechnology, Roland Institute of Pharmaceutical Sciences Berhampur-760010, Orissa, India

     

     
  • Rojita Mishra, Department of Biotechnology, Roland Institute of Pharmaceutical Sciences Berhampur-760010, Orissa, India

     

     
  • Kasturi Kumari, Department of Biotechnology, Roland Institute of Pharmaceutical Sciences Berhampur-760010, Orissa, India

     

     

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Published

2012-03-30

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Section

Articles

How to Cite

Anti-microbial and Free Radical Scavenging Activity of Chamomile Flower Essential Oil . (2012). Asian Journal of Pharmaceutical and Health Sciences, 2(1), 283-285. https://doi.org/10.5530/b8fa8382