Spectrophotometric Processing Data for Determination Ipragliflozine in Pure and in Tablet Dosage Form

Objective: This study aimed to develop three simple UV spectrophotometric methods for determination of ipragliflozin in bulk drugs and pharmaceutical dosage form in the presence of its oxidative degradation product. Methods: Manipulating ratio spectra were developed for the interested methods. The first method is an absorption factor using Zero-order absorption spectra of intact and its oxidative degradation product, the peak amplitudes of method were measured at 229 nm.The second method is the first drevitave using zero-order absorption spectra for derivatization and the peak amplitudes of method were measured at 257.8 nm. The third method is the ratio subtraction using 14 µg/mL of its oxidative degradation product as a divisor, finally the peak amplitudes of method were measured at 229 nm. Results and discussion: The calibration curve is linear over the concentration range of 2-14 µg/mL, the proposed methods were validated according to International Conference on Harmonization (ICH) guidelines and successfully applied for the determination of ipragliflozin in the presence of its oxidative degradation product. Conclusion: The proposed three methods are simple, rapid, economical, accurate and precise for determination of ipragliflozin in the presence of its oxidative degradation product.