Obesity is a physical condition that results from excessive storage of fat in the body. Weight more than 20% above the desired body mass index, which is calculated from an individualâ€™s age, height, and weight. The excess fat tissues result in obesity and causes health disorders. Poor diets and the lack of physical exercise are main factors in the increase of obesity. At present diet and physical exercise promote weight loss over short term, to achieve and maintain a healthy weight. To reduce the obesity protein powder is designed to be taken every day as nutritional supplement. By consuming protein throughout the day, to stabilize the blood sugar levels. Protein powder is mainly used for weight management to reducing body mass index. The present study aims to analyze the protein powder by FTIR spectroscopic technique, the pure protein powder was procured from a nutritional club and used as such. The FTIR spectra were recorded in the mid infrared region between 4000 â€“ 400 cm-1. A qualitative analysis of these protein powders has been carried out by making a satisfactory vibrational band assignment for the fundamental modes of vibration observed in the infrared spectrum. Some specific vibrations are identified; absorbance values are noted under different conditions of exposure. The ratios of absorbance among some specific modes of vibration are calculated, which represents the internal standards. The sets of the internal standards of the protein powder is compared with suitable storage conditions to check whether any change has taken place due to the environmental exposure.