Spectrophotometric Determination Of Nevirapine In Pharmaceuticals After Derivatization With 2,4-dinitro Phenylhydrazine

The aim of present work is to develop a simple and sensitive spectrophotometric method for nevirapine in pharmaceutical tablet dosage forms and to validate the method. The method is based on the interaction of nevirapine (keto group) with 2,4 dinitro phenyl hydrazine in acidic ethanol to form red colored hydrazone. The absorption maximum in the visible range of the spectrum, for the product was found to be 470 nm. The pH (3-4) and reaction time scan for the assay were optimized. Spectrophotometric method has been developed for the determination of nevirapine in nevirapine tablets. A linear relation of concentration (10-50 5g/mL) of nevirapine against absorbance at 470 nm was established. The method was successfully applied to the determination of nevirapine in tablet dosage form and the proposed method can be used for the routine determination of nevirapine in simple and complex dosage forms.