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Chronic Administration of D-galactose Induces Memory Loss, Cholinergic System Impairment and Oxidative Damage in Mice: Protective Effects of Piliostigma reticulatum Leaves Extract

Asian Journal of Pharmaceutical and Health Sciences,2018,8,3,1936-1943.
Published:September 2018
Type:Research Article
Author(s) affiliations:

Sidiki Neteydji1*,  S Pale2, KJS. Njapdounke1, GS Taiwe2, D Pahaye1, AK Kandeda3, E Ngo Bum4

1Department of Biological Sciences, Faculty of Science, University of Ngaoundere; Ngaoundere, Cameroon.

2Cameroon Department of Zoology and Animal Physiology- Faculty of Science, University of Buea, Buea, Cameroon.

3Department of Biological Science, High Training School, University of Yaounde I, Yaounde,  Cameroon.

4Institute of Mines and Petroleum Industries, University of Maroua, Cameroon.


Previous study showed that D-galactose (D-gal) increased production of Reactive Oxygen Species and resulted in impairment of memory and cholinergic system. Piliostigma reticulatum has been reported to have many benefits and medicinal properties. In this study, we evaluated the protective effect the aqueous extract of Piliostigma reticulatum against Dgalactose-induced memory loss, impairment of cholinergic system and oxidative damage. Our results displayed that Piliostigma reticulatum administration significantly improved behavioral performance of D-gal-treated mice in morris water maze task and open field test. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione content (GSH) in D-galactose-treated mice were enhanced, while the content of the lipid peroxidation product malondialdehyde (MDA) was decreased by the plant administration. Furthermore, our results also showed that Piliostigma reticulatum significantly inhibited cholinesterase (AchE) activity, increased the level of acetylcholine in the brain of D-gal-treated mice, which could help restore impairment of brain function.

Effects of D-gal and P. reticulatum on the spontaneous exploration of mice in the open field. (A) Number of crossing. (B) Number of grooming. (C) Number of rearing. Each value represents the mean ± ESM, n = 5, * p <0.05, ** p <0.01, *** p <0.001, significant difference from the negative control. ### p <0.001, significant difference from the neutral control. ANOVAfollowed by the Turkey test. CNe: Neutral control (distilled water). D-gal: Negative control (D-galactose (500 mg / kg)). PR: Piliostigma reticulatum