In mammals, kidneys are supplied by the right and the left renal artery and originate from the related side of the abdominal aorta. Renal arteries give rise to the dorsal and ventral branches and they were divided into the interlobar, arcuate, and interlobular arteries. The aim of present study was to compare and correlate the arterial segmentation of kidney in different mammals. In the present study 15 Kidneys (5 human kidney, 5 goat kidney and 5 buffalo) were taken. The comparison of the segmentation of renal artery in human and goat and buffalo were studied by using buteryte acetate as casting material. Kidney of goat having same segmental patterns as human kidney whereas in buffalo kidney is lobulated. Knowledge of the distribution of major branches of the renal artery is important in diagnosis and treatment of renal disease for surgeons. Variations in renal vascular anatomy have been important in increasing frequency of experimental renal transplantation.