Natural remedies from medicinal plants are considered to be effective and safe alternative treatment for liver toxicity. The present study was aimed to investigate the hepatoprotective activity of ethanolic extract of Desmodium oojeinense(Roxb.).H.Ohashi against Paracetamol induced Hepatotoxicity in rats. Ethanolic extract showed significant (p<0.05) hepatoprotective effect by lowering the serum levels of various biochemical parameters such as SGOT, SGPT, ALP, total Bilirubin and by increasing the levels of total Protein, in the selected model. These biochemical observations were in turn confirmed by histopathological examinations of liver sections and are comparable with the standard hepatoprotective drug Silymarin (100mg/kg bodyweight i.p.) which served as a positive control. Our findings suggested that the ethanolic extract of Desmodium oojeinense (Roxb.).H.Ohashi possesses the hepatoprotective activity.