The purpose of this research work is to determine the diuretic, saluretic, natriuretic, and carbonic anhydrase inhibition activity of hydroalcoholic extraction of seeds of Paspalum scrobiculatum Linn in Wistar albino rats. Wistar albino rats of either sex were divided into four groups. The control group received 0.1 mg/ml of CMC, the reference group receive frusemide (10 mg/kg) and the test group received 200 mg/kg and 400 mg/kg of hydroalcoholic extract of Paspalum scrobiculatum by oral route. The parameters measured for diuretic activity were urine volume and urinary concentration of electrolytes such as sodium, potassium and chloride. The crude hydroalcoholic extract of the plant at the dose 200 mg/kg and 400 mg/kg showed significant diuretic (p<0.01 and p<0.0001 respectively), saluretic (p<0.0001), and natriuretic activity (p<0.01 and p<0.0001 respectively). The diuretic index of the test group (200 mg/kg and 400 mg/kg) was 1.6822 ± 0.163 and 2.292 ± 0.1690 respectively. The present data about various parameters like urinary volume and electrolyte concentration (Na+, K+, and Cl-) indicate that Paspalum scrobiculatum has an ideal diuretic and natriuretic activity.