Plants are one of the foremost sources of natural medicine and a large number of modern drugs have been obtained from them.Garcinia mangostana L.is a tropical evergreen tree considered as “The queen of fruit” belongs to the family Guttiferae. Aim of the present study was to carry out the pharmacognostical, physico-chemical and phytochemical studies on the leaves of Garcinia mangostana L. This includes leaf morphology, microscopy, physicochemical parameters and phytochemical study. The purpose of this study was to explore anatomy, phytoconstituents present and fluorescence analysis of leaves of Garcinia mangostana L. Pharmacognostical and physico-chemical parameters help to develop standards and determine identity, purity and quality of the drug. Various primary and secondary metabolites present in the plant can be identified by preliminary phytochemical screening. Morphological study tells about the anatomical characters of the leaf and the phy¬tochemical analysis revealed the presence of alkaloids, carbohydrate, flavonoids, saponins, tannins, steroids and phenols. Soxhlet extraction using ethyl acetate as solvent shows maximum yield. Quantitative and fluorescence analysis were also performed. This study provides the pharmacognostical standards and phytochemical profile of Garcinia mangostana L.