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Development of a Validated RP-HPLC Method for Estimation of Quercetin

Asian Journal of Pharmaceutical and Health Sciences,2021,11,1,2415-2422.
Published:June 2021
Type:Original Article
Authors:
Author(s) affiliations:

Roma Mathew1,*,Joyamma Varkey2

1College of Pharmaceutical Sciences, Government Medical College, Thiruvananthapuram, Kerala, INDIA

2College of Pharmaceutical Sciences, Government Medical College, Thiruvananthapuram, Kerala, INDIA

Abstract:

The study aimed to develop a simple, accurate and reproducible method for the estimation of Quercetin using RP-HPLC.A C18 column(250 × 4.6 mm ID, 5µ particle size) with mobile phase consisting of 0.5% O-phosphoric acid: Methanol (50:50 v/v) was used. Quantitative evaluation was performed at 370 nm. The method was validated using ICH guidelines. The developed method is selective, precise and accurate and can be used for routine analysis of preparations in pharmaceutical industry quality control laboratories.

Standard curve of Quercetin by RP-HPLC