Development of New RP-HPLC Method for Simultaneous Estimation of Luliconazole and Naproxen Sodium in the Formulated Gel

T. Sree Ramya; Y. Padmavathi; Venkatesh Sama; Mallika Alvala; Ravi Alvala

Development of New RP-HPLC Method for Simultaneous Estimation of Luliconazole and Naproxen Sodium in the Formulated Gel

Asian Journal of Pharmaceutical and Health Sciences,2020,10,3,2364-2372.

Published:November 2020

Type:Original Article

Authors:

Author(s) affiliations:

T. Sree Ramya¹, Y. Padmavathi¹, Venkatesh Sama¹, Mallika Alvala³, Ravi Alvala¹*

¹G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad, Telangana, India- 500028.

²Founder and Instructor, MARS Training Academy, Dilsukhnagar, Hyderabad, Telangana, India-500 060.

Abstract:

The objective of the study was to develop a new simple, accurate, sensitive, reproducible RP-HPLC method for the simultaneous estimation of Luliconazole and Naproxen Sodium in formulated gel. The resolution of two drugs was achieved on enable C18 (4.6× 150mm, 5µm) column using mobile phase phosphate buffer pH 5.8 : methanol in the ratio 60:40v/v with isocratic elution, flow rate 1mL/min. Retention time of Luliconazole and Naproxen Sodium were 4.2 min and 6.8 min respectively, using analytical wavelength 220 nm. The percentage recovery for Luliconazole and Naproxen Sodium was found to be 97.8% and 99.3% respectively. The method was validated according to the ICH guidelines and it can be used for routine analysis in simultaneous estimation of luliconazole and naproxen sodium in pharmaceutical gel.

Keywords:HPLC, Luliconazole, Naproxen Sodium, Pharmaceutical Gel