Objective: Chronic bronchitis (CB) represent a classic phenotype of COPD having different genetic determinants. The overall prevalence of CB in India was found to be 3.49%. Methods: We assessed TNF-alpha polymorphism -238 (G/A) and risk of Chronic Bronchitis using PCR-RFLP. We studied 10 cases with chronic bronchitis and 10 healthy individuals. Results: GA genotype was found to be associated significantly with the increased risk of Chronic Bronchitis (p, 0.5); also G allele was displayed high risk of CB (76.9%) in our study population. In our study we also found elevated levels of IFN-gamma and 8 oxo-dG and decreased level of TNF-alpha in CB patients. Conclusion: Our study indicated, the presence of GA genotype being a risk factor in the development of CB in Central Indian Population.