Tumor Necrosis Factor Alpha Promoter -238 TNF-alpha (G/A) Locus Polymorphism and the Risk of Chronic Bronchitis

Asian Journal of Pharmaceutical and Health Sciences,2016,6,3,1503-1508.
Published:September 2016
Type:Research Article
Author(s) affiliations:

Protiti Bose1, Rashmi Bathri1, Jyoti Shrivastava2, Vikramsingh Gujar1, Lalit Kumar3

1Department of Research, Bhopal Memorial Hospital and Research Centre, Bhopal, Madhya Pradesh, India.

2Department of Biotechnology, APS University Rewa, Madhya Pradesh, India.

3Department of Pulmonary Medicine, BMHRC, Bhopal, Madhya Pradesh, India.


Objective: Chronic bronchitis (CB) represent a classic phenotype of COPD having different genetic determinants. The overall prevalence of CB in India was found to be 3.49%. Methods: We assessed TNF-alpha polymorphism -238 (G/A) and risk of Chronic Bronchitis using PCR-RFLP. We studied 10 cases with chronic bronchitis and 10 healthy individuals. Results: GA genotype was found to be associated significantly with the increased risk of Chronic Bronchitis (p, 0.5); also G allele was displayed high risk of CB (76.9%) in our study population. In our study we also found elevated levels of IFN-gamma and 8 oxo-dG and decreased level of TNF-alpha in CB patients. Conclusion: Our study indicated, the presence of GA genotype being a risk factor in the development of CB in Central Indian Population.


Avg. TNF-alpha conc. (ng/ml) with different genotypes in test and control subjects