Evaluation of the Effects of an Aqueous Extract of Schinus Terebinthifolius Involving Wild Bacterial Culture of Escherichia Coli

Asian Journal of Pharmaceutical and Health Sciences,2011,1,2,86-88.
Published:May 2011
Type:Short Communication

Stannous chloride (SnCl2 ) is the most used reducing agent in the marking of radiopharmaceuticals, but is highly toxic to cells, including cultures of Escherichia coli, because the formation of free radicals. Some plant extracts have protective actions against the damaging effect of SnCL2. We evaluate these damaging effects on wild culture of E. coli AB1157 (wild-type), and the effect of the agent together with an aqueous extract prepared from fresh leaves of Schinus terebinthifolius, already known as an extract help the healing process. Two experimental evaluations were conducted, evaluation of bacterial survival in liquid and solid medium. In the evaluation in liquid medium, aliquots of bacterial suspension was incubated with: (i) 0.9% NaCl, (ii) S. terebinthifolius extract, (iii)SnCl2 + S. terebinthifolius extract, (iv) SnCl2. After 60 min, aliquots of each culture was spred on petri dishes containing solid medium. In the evaluation of solid medium, plates containing solid medium prepared with bacterial culture for assessment of growth inhibition. Five discs of 6 mm were placed on each plate, and each was given samples of the following solutions: (i) 0.9% NaCl, (ii) S.terebinthifoliusextract (iii) S. terebinthifolius extract + SnCl2, (iv) SnCl2, (v) Amoxicillin + Clavulanic acid. After overnight incubation of the samples of the evaluation in a liquid medium, growth of cultures of E.coli was observed in the plates that cultures were incubated with a solution of SnCl with S. terebinthifolius extract. And after overnight incubation of samples of the evaluation in a solid medium, there was no inhibition zone where the disks were administered solution of SnCl2 along with S.terebinthifolius extract. This suggests that the extract has no toxicity over the E. coli cultures and protects cells from the damaging action of SnCl

Analysis of solid medium about the effects