The study reports an investigation of the microwave-assisted synthesis of gold nanoparticles (GNPs) using neem gum. The neem gum acts as both reducing and anti-agglomeration agents. The formation of GNPs was identified by instrumental analysis consists of UV-Vis spectrophotometry, Fourier-transform infrared (FTIR) spectrophotometry, X-ray diffraction (XRD), and transmission electron microscopy (TEM). The synthesized GNPs were found to be crystalline and spherical with an average size of 12±2 nm. These GNPs were assessed for the catalytic reduction of Methyl Orange (MO) and anti-bacterial activity. It was established that the reduction reaction follows the pseudo first order kinetics with a reaction rate constant of 0.212 min-1.