This study was carried out to investigate thyroid hormone TSH (Thyroid-stimulating Hormone), Free T3 (FT3) and Free T4 (FT4) status in non-pregnant women in Uttarakhand (India). Only 100 healthy pregnant women were selected for the present study. TSH, Free T3 (FT3) and Free T4 (FT4) were quantitatively analysed. FT3, FT4 and TSH in Euthyroid showed 6.19 ± 1.14 (pmol/L), 18.42 ± 4.50 (pmol/L), 2.02 ± 0.64 (mIU/L) respectively. Our study showed that 72, 6, 5, 2, 1, 10, 4 women were characterized into Euthyroid, Primary hypothyroid, Primary hyperthyroid, Secondary hypothyroid, Secondary hyperthyroid, Sub clinical hypothyroid and Sub clinical hyperthyroid respectively. Our study concluded that major non-pregnant women were healthy but 28% female were suffering from thyroid disease. Reference ranges of FT3, FT4 and TSH have been established for non-pregnant women of Uttarakhand by using 5th and 95th percentiles.